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To investigate the influence of the different sodium alginate concentration on the structure and property of hydrogel as scaffold for tissue engineering. With the same concentration of calcium chloride solution,different concentration of sodium alginate solution was mixed to prepare different types of hydrogels. The water content of hydrogel and gel mass fraction were determined,and their freeze dried microstructure changes were observed under the optical microscope. To obtain the hydrogel loading mesenchymal stem cells,the sterile sodium alginate solution and stem cells were mixed and reacted with calcium chloride solution. Then the survival cells released from the different hydrogels were observed using an inverted fluorescence microscopeation. Under the condition of certain concentration of calcium chloride,the network structure of hydrogel was becoming looser and easier for stem cells to survive and release,with the decrease of the concentration of sodium alginate. It could be concluded that sodium alginate hydrogel as tissue engineering scaffold had a good prospect for nerve injury repair application.
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Basic Information:
China Classification Code:R318.08
Citation Information:
[1]ZHANG Kun,MA Shanshan,MENG Nan ,et al.Effect of Sodium Alginate Concentration on the Structure and Properties of a New Scaffold for Nerve Tissue Engineering[J].Journal of Zhengzhou University(Natural Science Edition),2015,47(04):99-102.
Fund Information:
河南省博士后科研项目,编号2014020
2016-01-11
2016-01-11
2016-01-11